Figure 3.

Genotype analysis of progeny of chimeric mice derived from ES cells with chromosomal rearrangements. Mouse tail DNA obtained from two litters of progeny of chimera Vc1 was analyzed by PCR, using primers designed to amplify a fragment of (from Top to Bottom) the PGK-Puro junction, the modified Idd allele on its 3′ end or its 5′ end, the Hyg–Neo junction, and the modified Arvcf allele on its 5′ end (indicated, respectively, as fragments D, A, B, E, and C in Fig. 1b). Mice positive for PCR fragments D and A contain a chromosome with an interstitial deletion, whereas mice positive for PCR fragments B, E, and C contain a chromosome with an interstitial duplication.