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Antibiotic resistance and PCR analysis of clones obtained from transfections of doubly targeted ES cell lines with Cre expression plasmids
| Idd–Arvcf knockout cell line | No. of clones
|
|||||
|---|---|---|---|---|---|---|
| CMV-Cre
|
CAG-Cre
|
|||||
| 1c1, 3c4, 3b3 | 4c1 | 4b1 | 4b4 | 3c1 | 4d1 | |
| PuroR | 0 | 84 | 3 | 5 | 5 | >1,000 |
| HygR, NeoR | 0 | 0/24 | 2/2 | 1/1 | NT | 0/42 |
| PGK-Puro PCR+ | 0 | 24/24 | 2/2 | 1/1 | NT | 41/42 |
| Hyg-Neo PCR+ | 0 | 0/24 | 2/2 | 1/1 | NT | 0/42 |
NT, not tested.
