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Sec7-DsRed partially colocalizes with Apl5-GFP but not with GFP-HOPS subunits. Cells expressing Sec7-DsRed and (A) Apl5-GFP or (B) GFP-HOPS subunits were analyzed by fluorescence microscopy. Bar, 2 μm. See Supplemental Movies 4–7.