Table 1.
Strains
| Strain | Genotype | Source |
|---|---|---|
| BY4742 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | ATCC |
| BY4742 vam3Δ | BY4742; vam3Δ::KanMX4 | Invitrogen |
| BY4742 vps8Δ | BY4742; vps8Δ::KanMX4 | Invitrogen |
| BY4742 vps16Δ | BY4742; vps16Δ::KanMX4 | Invitrogen |
| BY4742 vps39Δ | BY4742; vps39Δ::KanMX4 | Invitrogen |
| BY4742 vps41Δ | BY4742; vps41Δ::KanMX4 | Invitrogen |
| BY4742 ypt7Δ | BY4742; ypt7Δ::KanMX4 | Invitrogen |
| AMY969 | BY4742; VPS16-GFP::HIS3 APL5-mCherry::HphMX | This study |
| AMY1246 | BY4742; APL5-mCherry::HphMX pRS415-NOP1pr-GFP-VPS41 | This study |
| AMY1244 | BY4742; VPS16-GFP::HIS3 pRS316-SEC7-DsRed | This study |
| AMY1248 | BY4742; pRS415-NOP1pr-GFP-VPS41 pRS316-SEC7-DsRed | This study |
| AMY632 | BY4742; APL5-mCherry::HphMX | This study |
| SEY6210 | MATα leu2-3,112 ura3-52 his3-Δ200 trp1-Δ901 lys2-801 suc2-Δ9 | Robinson et al. (1988) |
| AMY1252 | SEY6210; APL5-ttx-GFP::TRP1 | This study |
| AMY642 | SEY6210; vam3tsf APL5-ttx-GFP::TRP1 | This study; Darsow et al. (2001) |
| BJ3505 | MATα pep4::HIS3 prb1-Δ1.6R his3-Δ200 lys2-801 trp1-Δ101 (gal3) ura3-52 gal2 can1 | Jones et al. (1982) |
| BJ3505 GFP-Vps39 | BJ3505; vps39Δ::URA3 pRS404-GFP-VPS39 | Wang et al. (2002) |
| AMY971 | BJ3505; vps39Δ::URA3 pRS404-GFP-VPS39 APL5-mCherry::HphMX | This study |
| AMY1192 | BJ3505; VPS33-ttx-GFP::TRP1 APL5-mCherry::HphMX | This study |
| AMY1250 | BJ3505; VPS33-ttx-GFP::TRP1 pRS316-SEC7-DsRed | This study |
| AMY1205 | BJ3505; APL5-ttx-GFP::TRP1 | This study |
| AMY1251 | BJ3505; APL5-ttx-GFP::TRP1 pRS316-SEC7-DsRed | This study |
| AMY1247 | BJ3505; vps41Δ::KanMX4 APL5-ttx-GFP::TRP1 pRS316-SEC7-DsRed | This study |
| AMY1278 | BJ3505; vps1Δ::KanMX4 APL5-ttx-GFP::TRP1 pRS316-SEC7-DsRed | This study |