Table 1.

Dissociation and Association Rate Constants of Wild-Type RT^a

method	protein solnsb	kdiss, 10−6 s−1	kobs, 10−6 s−1	kass, M−1s−1
dissociation reaction
pre-steady-state assay	50 nM p66/p51	4.4 ± 0.2
Trp fluorescencec	50 nM p66/p51	3.9 ± 0.3
association reaction
pre-steady-state assay	0.5 µM p66, 0.5 µM p51		5.8 ± 0.2	2.2 ± 0.2 d
	0.5 µM p66, 2.5 µM p51		7.8 ± 0.3	1.3 ± 0.2 e
				1.4 ± 0.2 f
Trp fluorescencec	0.5 μM p66, 0.5 μM p51		5.4 ± 0.4

^aRT buffer D with 1 mM TCEP, 5 °C. Errors are standard deviations of 3–9 experiments

^bFinal concentrations

^cλ_ex = 295 nm, λ_em = 340 nm

^dCalculated from eq 3

^eCalculated from eq 4

^fCalculated from eq 13.