Table 2.

Dissociation and Association Rate Constants of Labeled RT^a

protein solnsb	kdiss, 10−6 s−1	kobs, 10−6 s−1	kassc, M−1s−1
dissociation reaction
50 nM p66-A488/p51-QSY9	2.5 ± 0.5
50 nM p66-QSY9/p51-A488	1.9 ± 0.4
association reaction
0.5 µM p66-A488, 0.5 µM p51-QSY9		4.1 ± 0.4
0.5 µM p66-QSY9, 0.5 µM p51-A488		3.4 ± 0.4
0.5 µM p66-A488, 1.5 µM p51-QSY9		4.8 ± 0.3	1.6 ± 0.2
0.5 µM p66-A488, 2.0 µM p51-QSY9		5.7 ± 0.4	1.6 ± 0.2
0.5 µM p66-A488, 2.5 µM p51-QSY9		7.1 ± 0.3	1.8 ± 0.4

^aRT buffer D, 5 °C. Errors are standard deviations of 3 experiments. λ_ex = 450 nm, λ_em = 520 nm

^bFinal concentrations

^cCalculated from eq 13.