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. 2009 Nov 13;5(11):e1000720. doi: 10.1371/journal.pgen.1000720

Figure 6. erbB2 promotes EMT in the epidermis of pen/lgl2 mutant larvae.

Figure 6

DIC images of the morphology of wild-type (A), lgl2,erbB2+/− (B) and lgl2,erbB2−/− (C) larvae at 108hpf. Keratin (D–F), E-cadherin (G–I), BrdU (J–L) and mmp9 (M–O) staining in wild-type (D, G, J, M), lgl2,erbB2+/− (E, H, K, N) and lgl2,erbB2−/−(F, I, L, O) larvae at 108hpf. The DIC images of the morphology reveals that cellular clumps, a typical characteristic of pen/lgl2 mutant larvae are present in lgl2,erbB2+/− (B) but are absent in lgl2,erbB2−/−(C). The keratin, E-cadherin and mmp9 staining in lgl2,erbB2−/− (F, I, O) appear similar to wild-type (D, G, M) indicating erbb2 promotes the EMT phenotype in pen/lgl2 mutant larvae. Note that, in lgl2,erbB2−/− larvae (L) BrdU incorporation does not decrease to wild-type levels (J).