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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Angew Chem Int Ed Engl. 2009;48(37):6790–6810. doi: 10.1002/anie.200900231

Figure 7.

Figure 7

Top: The flow-through mode setup consisting of a source point and a waste collector for solutions flowing in and out of the SPR system, a peristaltic pump, and personal computer (PC) for collecting data and offline analysis. Phage is immobilized on the gold-coated SPR sensor chip in the system. Bottom: Typical dose/response curves obtained from a phage-immobilized SPR sensor in the flow-through mode. Curve A represents the responses obtained from the β-gal-specific phage 1G40 immobilized in one of the two channels of the sensor against graded concentrations of β-gal (0.0032—210 nM). Curve B represents the responses obtained from the wild-type phage F8-5 immobilized in the second channel. Even at the highest concentration of β-gal tested (210 nM), the response obtained from the β-gal-specific phage is approximately ten times greater than that obtained from the wild-type phage.[110] RU = resonance units.