Table 1.

Nuclear dynamics of combinations of influenza proteins.

Transfected plasmids	Total n (exp)a	t1/2 (s) (mean ± s.d.)	rel. to P/2P	p value t-test different to bP/2P	DC (μm2/s) (mean ± s.d.)	rel. to P/2P	p value t-test different to bP/2P
PB2-GFP	55 (5)	0.93 ± 0.83	1	1	0.91 ± 0.62	1	1
PB2-GFP, PA	22 (2)	0.86 ± 0.51	0.92	0.72	0.88 ± 0.53	0.97	0.87
PB2-GFP, PB1	30 (3)	1.44 ± 0.90	1.55	0.01	0.56 ± 0.40	0.62	7.6 × 10− 3
PB2-GFP, PB1, PA	64 (6)	7.44 ± 5.28	8.00	9.0 × 10− 16	0.11 ± 0.07	0.12	3.8 × 10− 18
PB2-GFP, PB1-SDD,PA	68 (6)	8.92 ± 5.88	9.59	1.9 × 10− 18	0.09 ± 0.07	0.10	1.0 × 10− 20
PB2-GFP, PB1, PA, NP	22 (2)	8.92 ± 6.39	9.59	1.7 × 10− 14	0.08 ± 0.04	0.09	2.6 × 10− 8
PA-GFP, PB1	20 (2)	1.05 ± 0.81	1	1	1.13 ± 1.18	1	1
PA-GFP, PB2	24 (2)	0.88 ± 1.75	0.84	0.69	1.49 ± 1.06	1.32	0.29
PA-GFP, PB1, PB2	20 (2)	2.34 ± 1.39	2.23	9.7 × 10− 4	0.32 ± 0.19	0.28	4.3 × 10− 3
PA-GFP, PB1-SDD, PB2	24 (2)	2.49 ± 1.69	2.37	1.2 × 10− 3	0.31 ± 0.20	0.27	1.6 × 10− 3
PB1-GFP, PA	31 (3)	1.17 ± 0.85	1	1	0.87 ± 0.82	1	1
PB1-GFP, PA, PB2	32 (3)	6.02 ± 4.20	5.14	3.5 × 10− 8	0.13 ± 0.10	0.15	5.2 × 10− 6
PB1-GFP, PA, PB2-F363A	33 (3)	1.53 ± 0.91	1.31	0.1	0.49 ± 0.31	0.56	0.02
PB1-GFP, PA, PB2-F404A	34 (3)	5.97 ± 3.48	5.10	3.1 × 10− 10	0.12 ± 0.08	0.14	1.9 × 10− 6

^aTotal number of cells analysed from (in parentheses) the indicated number of independent transfections.

^bP indicates PB2-GFP; 2P, PB1-GFP + PA or PB1 + PA-GFP as appropriate.