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. 2009 Aug 27;28(20):3117–3131. doi: 10.1038/emboj.2009.252

Figure 3.

Figure 3

Localization of Rng2 and its truncated proteins. All constructs were expressed in wild-type cells from plasmids under the control of the strongest nmt1 promoter. We observed cells that expressed the GFP fusions before the effects of OE became apparent. Images of (A) GFP-Rng2, (B) GFP-Rng2Ns, (C) GFP-Rng2ΔNs, and (D) GFP-Rng2CHD. (E–G) Localization of GFP-Rng2, GFP-Rng2ΔNs, and GFP- Rng2Ns in the absence of F-actin. Cells expressing each construct were treated with 0.2% DMSO alone (–Lat-A) or with 0.2% DMSO plus 4 μM Lat-A (+Lat-A) for 10 min at 25°C, before being stained for DNA. (H) Images of GFP-Rng2 or GFP-Rng2ΔNs expressed at a low level in the presence of 5 μM thiamine (repressed conditions). (I) Cells bearing pREP1-GFP-Rng2ts were grown under repressed conditions at 25°C, shifted to 25 or 36°C for 30 min, and stained for DNA. Arrowheads and small bars indicate the CR and bands of dots, respectively. Double arrowheads indicate the CR during contraction. (J) Summary of the localization of Rng2 constructs. Bars: 5 μm.