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. 2009 Sep 10;28(20):3074–3089. doi: 10.1038/emboj.2009.255

Figure 5.

Figure 5

Drp1 is required for separation during transient fusion. (A) Effect of Drp1 domain negative or Drp1YFP overexpression on mitochondrial morphology. H9c2 cells were transfected with mtRFP, mtRFP/Drp1K38ACFP, and mtRFP/Drp1YFP. (B) Effect of Drp1 domain negative or Drp1YFP overexpression (OE) on mitochondrial fusion quantity and quality. Statistically significant differences from the control are marked by an asterisk (P⩽0.04). (C) Effect of Drp1 domain negative or Drp1YFP overexpression on durations of transient fusion. (D) The time course of Drp1YFP fluorescence involved in mitochondrial transient fusion. At 0 s the photobleaching of mtRFP fluorescence is finished. (E) Transient fusion and Drp1YFP fluorescence time chart for individual mitochondria.