Figure 2.

Re-initiation supporting protein (RISP) and transactivator viroplasmin (TAV) accumulate in polyribosomes during viral infection. (A, B) Ribosomal profiles of polyribosomes and ribosomal species from healthy (left) and Cauliflower mosaic virus (CaMV)-infected (right) turnip plants (A) untreated and (B) treated with 30 mM EDTA. A, B show the UV profile of the gradient with 40S and 60S, monosomes (80S) and polysomes indicated are shown. 1 ml aliquot fractions were either precipitated with 10% TCA and analyzed by SDS–PAGE and immunoblotting using polyclonal antibodies against TAV and RISP (lower panels); or analyzed by agarose gel electrophoresis (upper panel in B). Positions of 18S and 28S rRNAs are indicated. (C) Immunoblotting using polyclonal antibodies against RISP of extracts isolated from Arabidopsis (At), healthy (Turnip −) and CaMV-infected (Turnip +), turnip plants and recombinant RISP expressed in Escherichia coli. Loading controls are shown (lower panel).