Stable MCF7 cell clones transfected with vector control (V(C)), CEACAM1-4L wild type (4L), T457D (TD), T457A (TA), S461D (SD), S461A (SA), T457D+S461D (DD), and T457A+S461A (AA) were grown in 12-well plates and were stained with CEACAM1 mAb 5F4 followed by Alexa-488 conjugated rabbit anti-murine IgG staining. Cells were analysed by inverted fluorescence microscope. (Magnification 100×)