Stable MCF7 cell clones transfected with vector control (V(C)), CEACAM1-4L wild type (4L), T457D (TD), T457A (TA), S461D (SD), S461A (SA), T457D+S461D (DD), and T457A+S461A (AA) were grown in Matrigel Sandwich for 10 days and were stained for F-actin with Texas Red-conjugated phalloidin. Cells were analysed by inverted fluorescence microscope. (Magnification 200×)