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Detection of ECA in the LPS preparations of P. mirabilis strains. Panel A. Western blotting using 1:200 diluted monospecific anti-E. coli O14:K7 antiserum enriched in ECA-specific antibodies. Panel B. Silver-stained gel after transfer of the samples to the PVDF membrane. The LPS Ph/W and LPS PCP preparations (15 µg per lane) of P. mirabilis strains O28, R4/O28, S1959, and R110/1959 and ECAPG of S. montevideo (positive control, 2 µg per lane) were analyzed as indicated below the images.
