Figure 3.

Protein expression of PKR in non-stimulated and IFN-α stimulated beta TC3 and alpha TC3 cells. After beta TC3 and alpha TC3 cells were stimulated with 500 U/mL (lane 2 and 5) and 2000 U/mL (lane 3 and 6) of IFN-α for 24 hours, prepared lysates were subjected to 12% SDS-PAGE, transferred onto nitrocellulose, and immunostained with a polyclonal antibody specific for human PKR. Two protein bands were detected; the p48 subunit is the degradation product of PKR (p68). The relative levels of protein were determined by quantifying the immunoblots using the Quantity One 1-D Image software (see Table 1).