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Protein expression and activity of 2′5′AS in beta TC3 and alpha TC3 cells transfected with mouse IFN-α4-containing vector and poly(I:C). Beta TC3 and alpha TC3 cells were transiently transfected with pcDNA3.1-mIFN-α4, poly(I:C), and pcDNA3.1-mIFN-α4 plus poly(I:C), respectively. pcDNA3.1 empty vector was used as a negative control. After 24 hours, cell lysates were prepared. Immunoblot (a) and 2′5′AS activity assay (b) were performed as described under the materials and methods.
