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. 2009 Oct 29;2009:631026. doi: 10.1155/2009/631026

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Copyright © 2009 M. Li et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Induction of apoptosis in beta TC3 and alpha TC3 cells after transfection of IFN-α4-containing vector and poly(I:C). After beta TC3 and alpha TC3 cells were transiently transfected with pcDNA3.1-mIFN-α4, poly(I:C), and pcDNA3.1-mIFN-α4 plus poly(I:C) for 24 hours, respectively, induction of apoptosis was detected by immunoblots. pcDNA3.1 empty vector was used as a negative control. Cells lysates were separated on 15% SDS-PAGE and transferred to nitrocellulose membranes. The polyclonal antibody specific to mouse caspase 3 was used to detect both the intact as well as the cleaved caspase 3 proteins.