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. 2009 Oct 28;2009:829243. doi: 10.1155/2009/829243

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Copyright © 2009 Yiyao Liu et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Transmembrane invasion of HeLa cells through PET membrane precoated with gelatin. (a) representative images of HeLa cells invasion (labelled into green) and (b) the statistical data of invaded HeLa cell numbers in various conditions. The numbers of invaded HeLa cells were quantified by cell counting from more than five micrographic fields using magnification of 10×. Pl: in the presence of platelets; Pl+Th: in the presence of thrombin-activated platelets; Pl+Ep: thrombin-activated platelets treated with eptifibatide; RGDWE: HeLa cells were treated with RGDWE peptides to block αvβ3 integrin. *P < .05 (Pl+Th) versus Pl or (Pl+Ep); ^#P < .05 Pl versus control. Control is considered as the sample of equal volume of cell culture medium added.