Skip to main content
. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Trends Biotechnol. 2008 Mar 4;26(4):181–189. doi: 10.1016/j.tibtech.2007.12.009

Figure 2.

Figure 2

Potential of human mesenchymal stem cells to form osteochondral grafts. hMSCs can be isolated from the patient’s tissue, expanded in culture and used to form osteochondral composites. In one notable example, hMSCs were chondrogenically induced in pellets (medium supplemented with dexamethasone, L-ascorbic acid and transforming growth factor-β) and press-coated on porous polylactic blocks [30]. Confluent monolayers of the same cells were osteogenically induced (culture medium supplemented with dexamethasone, L-ascorbic acid and β-glycerophosphate) and seeded into the porous regions of the scaffolds from the opposite side. (a) Osteochondral composites with a 2 mm thick cartilaginous layer (CL) integrated with the underlying osteogenic layer (OL) were formed by cultivation in medium containing both chondrogenic and osteogenic factors. Scale bar represents 1.5 mm. (b) Deposition of cartilaginous matrix (blue glycosaminoglycans stain) was limited to the CL. Arrows indicate the boundary between CL and OL. (c) Mineralized matrix (red stain) was limited to the osteogenic layer. Images were reproduced with permission from [30].