Figure 3.
Strategy for immobilization of proteins containing cell-adhesive ligands. The fibronectin 10th type III domain is fused to cutinase, which specifically binds a suicide ligand of the substrate to give covalent immobilization of proteins while maintaining activity and orientation. An optical micrograph of swiss 3T3 cells adherent to the model substrate (upper right). The cells displayed normal cytoskeleton and focal adhesion structures (lower right).