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Duct formation in the developing SMG. E13.5 glands were immunostained with an antibody to the duct-specific marker, cytokeratin 7 (K7), and counterstained for F-actin with rhodamine-phalloidin (red). Intense regions of F-actin localization overlapped with K7 staining (filled arrows) confirming identification of these actin-rich areas as ductal structures. Scale bar = 10 μm.
