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Presumptive duct cells have prominent E-cadherin and ZO-1 staining at their apical domains. A,B: Confocal imaging of E13.5 glands revealed the distal region of the initial stalk with multilayered cuboidal cells and with focused E-cadherin at the apical and lateral cell borders of the innermost cell layer (A, block arrow) facing the central lumen. B: ZO-1 was detected at the apical cell–cell borders of the presumptive ducts (B, block arrow) facing the central lumen. Scale bar = 10 μm.
