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. Author manuscript; available in PMC: 2010 Jul 15.
Published in final edited form as: Cancer Res. 2009 Jun 23;69(14):5673–5680. doi: 10.1158/0008-5472.CAN-08-4512

Fig. 2.

Fig. 2

Partial silencing of DPAGT1 enhances intercellular adhesion in A253 cells. (A). Effects of DPAGT1 siRNA (S) on DPAGT1 expression in NS and S cells. DPAGT1 transcript levels were measured by real-time PCR, and protein levels were determined by Western blot. Bargraph, Fold change of DPAGT1 abundance in S cells in comparison with NS cells after normalization to actin. (B) Western blot of associated proteins in E-cad immunoprecipitates from NS and S cells. Bargraph, Fold changes in expression levels in S cells in comparison to NS cells after normalization to E-cad. (C) Immunofluorescence localization of E-cad in NS and S cells. Cells were counterstained for F-actin with rhodamine-phalloidin to assess cytoarchitecture. Size bars: 20 µm. All studies represent results from three different experiments (*P<0.01; **P<0.005; ***P<0.001).