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. Author manuscript; available in PMC: 2010 Aug 14.
Published in final edited form as: J Mol Biol. 2009 Jun 3;391(2):327–340. doi: 10.1016/j.jmb.2009.05.081

Figure 2.

Figure 2

Spectrophotometric studies probing for a flavin-derived iminium ion. Spectra were recorded as 0–4 mM UDP-Galp (a) or 0–10 mM UDP-Glc (b) was titrated into a sample of reduced UGM. The red line represents the absorbance spectrum at the highest concentration of ligand. Inset shows absorbance at 500 nm, the wavelength of iminium ion absorbance. A change is detected for UDP-Galp but not UDP-Glc. Results in (a) have been published previously25 and are shown for comparison.