Table II.
ATPase Activity of Wild-Type and Hybrid Enzymes
Specific activity (μmol/min/mg) for production of ADP in the presence of various substratesa |
|||||||
---|---|---|---|---|---|---|---|
Enzyme | ATP | ATP HCO3− | ATP AIR | ATP AIR HCO3− | ATP HCO2− | ATP GAR | ATP GAR HCO2− |
PurT | 0.021 | 0.031 | 0.033 | 0.030 | 0.16 | 4.64 | 5.14 |
PurK | 0.23 | 1.09 | 8.72 | 10.0 | 0.35 | 0.46 | 0.40 |
KATBC | 0.45 | 0.40 | 0.39 | 0.41 | 0.47 | 0.54 | 0.61 |
TAKBC | 0.66 | 0.69 | 0.91 | 0.96 | 0.65 | 0.61 | 0.67 |
TACKB | 0.073 | 0.073 | 0.087 | 0.13 | 0.061 | 0.10 | 0.099 |
KACTB | 0.028 | 0.026 | 0.053 | 0.077 | 0.022 | 0.033 | 0.037 |
KABTC | 0.16 | 0.15 | 0.14 | 0.14 | 0.14 | 0.10 | 0.12 |
TABKC | 0.26 | 0.23 | 0.25 | 0.27 | 0.20 | 0.22 | 0.28 |
Specific activities were determined by measuring ATPase activities under saturating concentrations of each substrate. The standard error for each value is less than 20%.