Table III.
Production of Acyl Phosphates by Wild-Type and Hybrid Enzymes
| Specific activity (μmol/min/mg)×10−4 for production of acyl phosphates using various substratesa |
|||
|---|---|---|---|
| Enzyme | Acetyl phosphate | Formyl phosphate | Carboxyl phosphate |
| PurTb | 4.4 × 103 | NAc | NA |
| PurTd | 715 | NDe | ND |
| PurKd | ND | ND | ND |
| KATBCd | 3.8 | 2.8 | ND |
| TAKBCd | 8.6 | 4.0 | ND |
| TACKBd | 11.1 | 1.7 | ND |
| KACTBd | 0.61 | 0.43 | ND |
| KABTCd | 10.7 | 7.7 | ND |
| TABKCd | 0.80 | 0.75 | ND |
a
Specific activities were determined under saturating concentrations of each substrate. The standard error is less than 10%.
b
Adapted from Ref.24.
c
NA not applicable.
d
Fusion protein with an N-terminal MBP domain.
e
ND not detected.