Table I.

The Optimized Recipe of the C-750501 Minimal Medium for Autoinduction and the Optimized High Cell Density Minimal Medium for Triple-Labeling Proteins

Optimized autoinduction minimal medium	Optimized high-cell-density IPTG-induction minimal medium
50 mM Na2HPO4	50 mM Na2HPO4·7H2O
50 mM KH2PO4 (pH 8.0–8.2)	25 mM KH2PO4 (pH 8.0–8.2)
50 mM15NH4Cl	10 mM NaCl
5 mM Na2SO4	5 mM MgSO4
2 mM MgSO4	0.2 mM CaCl2
0.5–22× metals	0.25× Metals
(Based on Studier's recipe)	0.25× Vitamins
0.4% 13C-glycerol	0.1% NH4Cl or 15NH4Cl
0.05% 13C-glucose	1.0% Glucose or 13C-Glucose
0.01% ß-lactose
12× Vitamins
(100 × BME vitamins stock solution, SIGMA, MO)