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Ponceau-S staining (left) and chemiluminescent (right) images of human and rat hemoglobin treated with the biotinylated aldehyde. “M” denotes biotinylated protein markers. In the right image, chemiluminescence intensity indicates the amount of N-Hcy proteins; in the left image, the color density from Ponceau-S staining indicates the total amount of protein. On each image, rat proteins (60 μg) are shown in the right lane and human hemoglobin (60 μg) in the left lane.
