Table 2.
Differential protein expression in mouse uterus after E2 treatment identified by spectral counting
| Protein Name | IPI accession number |
Number of unique peptides |
Sequence coverage (%) |
Average normalized spectral count ± standard deviation,a Control |
Average normalized spectral count ± standard deviation,a E2- treated |
P-value from G testb |
Fold change |
|---|---|---|---|---|---|---|---|
| Eif4a1 Eukaryotic initiation factor 4A-I |
IPI00118676 IPI00761992 |
3 | 13 | 0 | 3 ± 1 | 0.0005 | UEc |
| Krt19 Keratin, type I cytoskeletal 19 |
IPI00112947 | 10 | 36 | 1 ± 1 | 16 ± 3 | 3E-15 | >10 |
| Eef2 Elongation factor 2 |
IPI00466069 | 4 | 8.4 | <1 | 5 ± 1 | 7E-05 | >10 |
| Flna Isoform 1 of Filamin-A |
IPI00131138 IPI00830793 |
22 | 15 | 3 ± 1 | 30 ± 8 | 4E-24 | 10 |
| Rplp1 60S acidic ribosomal protein P1 |
IPI00113377 | 3 | 80 | 2 ± 1 | 10 ± 1 | 7E-06 | 5.0 |
| Hspa5 78 kDa glucose- regulated protein precursor |
IPI00319992 | 7 | 17 | 2 ± 1 | 9 ± 1 | 7E-06 | 4.5 |
| Cnn1 Isoform Alpha of Calponin-1 |
IPI00116645 IPI00228258 |
3 | 13 | 1d | 4 ± 1 | 0.003 | 4.0 |
| Atp5a1 ATP synthase subunit alpha, mitochondrial precursor |
IPI00130280 IPI00857439 |
3 | 10 | 1d | 4 ± 1 | 0.01 | 4.0e |
| Eef1a1 Elongation factor 1-alpha 1 |
IPI00307837 | 4 | 18 | 3 ± 2 | 9 ± 1 | 0.0003 | 3.0 |
| Des Desminf | IPI00130102 | 15 | 45 | 25 ± 10 | 54 ± 7 | 2E-11 | 2.2 |
| Hist1h2bf Histone H2B | IPI00114642 IPI00134534 IPI00227930 IPI00265768 IPI00282266 IPI00282269 IPI00348270 IPI00461514 IPI00554853 IPI00648991 IPI00761713 |
2 | 20 | 20 ± 4 | 31 ± 2 | 0.002 | 1.6 |
| Myl6 Isoform Smooth muscle of Myosin light polypeptide 6 |
IPI00354819 IPI00409817 |
7 | 59 | 36 ± 7 | 22 ± 4 | 0.0002 | 0.6 |
| Tln1 Talin-1 | IPI00465786 | 10 | 8.9 | 10 ± 4 | 4 ± 2 | 0.006 | 0.4 |
| Arhgdia Rho GDP- dissociation inhibitor 1 |
IPI00322312 | 3 | 31 | 11 ± 2 | 4 ± 1 | 0.0003 | 0.4 |
| Lum Lumican precursor | IPI00313900 | 5 | 25 | 20 ± 2 | 7 ± 1 | 6E-07 | 0.4 |
| Calm3;Calm2;Calm1 Calmodulin |
IPI00761696 | 3 | 31 | 14 ± 2 | 5 ± 1 | 1E-05 | 0.4 |
| Vcl Vinculin | IPI00405227 | 11 | 19 | 23 ± 5 | 7 ± 1 | 7E-09 | 0.3 |
| Park7 Protein DJ-1 | IPI00117264 | 4 | 37 | 7 ± 2 | 2 ± 1 | 0.002 | 0.3 |
| Tpi1 Triosephosphate isomerase |
IPI00467833 | 4 | 29 | 12 ± 1 | 4 ± 1 | 2E-05 | 0.3 |
| Ptrf Polymerase I and transcript release factor |
IPI00117689 | 4 | 15 | 14 ± 3 | 4 ± 1 | 1E-06 | 0.3 |
| Hnrnpa2b1 Isoform 3 of heterogeneous nuclear ribonucleoproteins A2/B1 |
IPI00405058 IPI00622847 IPI00828488 IPI00853914 |
3 | 19 | 5 ± 2 | 1 ± 1 | 0.0004 | 0.2 |
| Ogn Mimecan precursor |
IPI00120848 | 3 | 12 | 16 ± 3 | 3 ± 1 | 8E-10 | 0.2 |
| Anxa6 annexin A6 | IPI00310240 IPI00554894 IPI00649152 |
6 | 16 | 7 ± 1 | 1 ± 1 | 0.0001 | 0.2 |
| Pebp1 Phosphatidylethanol- amine-binding protein 1 |
IPI00137730 | 5 | 58 | 10 ± 2 | 1 ± 1 | 5E-08 | 0.1 |
| Gstm1 Glutathione S- transferase Mu 1 |
IPI00230212 IPI00649135 |
3 | 20 | 7 ± 2 | 0 | 4E-08 | UCg |
| Prelp Prolargin precursor |
IPI00122293 | 2 | 6.9 | 5 ± 2 | 0 | 2E-06 | UCg |
| Prdx6 Peroxiredoxin-6 | IPI00555059 IPI00758024 |
3 | 26 | 4 ± 1 | 0 | 3E-05 | UCg |
| Fabp4 Fatty acid- binding protein, adipocyte |
IPI00116705 | 2 | 20 | 3 ± 2 | 0 | 0.0002 | UCg |
a
N=4 (uterine tissue from four different animals)
b
Spectral counts from each animal in control and E2-treated groups were subjected to a summation-based G statistical test (P<0.05).
c
Unique in E2-treated animals.
d
Standard deviation <0.5
e
Validated by Western blotting (Supplementary Figure 2b online).
f
Protein grouping ambiguity reported by Scaffold was resolved by XIC quantitation of sequence-specific peptide
g
Unique in control (vehicle-treated) animals.