Table 2.
Strain and plasmids used in this study.
| A. Strains | Relevant Genotype | Source or Derivation |
|---|---|---|
| CAG12072 | sfsB203::Tn10 | Singer et al., 1989 |
| MG1655 | K-12 wild-type rph-1 | Blattner et al., 1997 |
| STL7679 | [pSTL 346] | Cmr transformation into MG1655 |
| STL7742 | obgE::Tn5 | Foti et al., 2005 |
| STL8248 | F- ompT hsdS(rB-mB-) dcm- Tetr gal λ (DE3) [pJT130] |
Tan et al., 2002 |
| STL8783 | relA251::kan | Metzger et al., 1989 |
| STL8789 | obgE::Tn5 sfsB203::Tn10 | Kmr tranductant P1 STL7742 X STL519 |
| STL8813 |
obgE::Tn5 relA251::kan sfsB203::Tn10 |
Tcr transductant P1 STL8789 X STL8783 |
| STL12512 | [pMK8] | Apr transformation into MG1655 |
| STL12758 | pstA::parS | Ferullo & Lovett,2008 |
| STL12760 | gadB::parS | Ferullo & Lovett, 2008 |
| STL13897 | obgE::Tn5 [pSTL 346] | Cmr transformation into STL 7742 |
| STL14013 | obgE::Tn5 [pMK8] | Apr transformation into STL7742 |
| STL14030 |
obgE::Tn5 pstA::parS sfsB203::Tn10 |
Tcr transductant P1 STL8789 X STL12758 |
| STL14033 |
obgE::Tn5 gadB::parS sfsB203::Tn10 |
Tcr transductant P1 STL8789 X STL12760 |
| B. Plasmids | ||
|---|---|---|
| pBAD33 | cat araC | Schreiber et al., 1991 |
| pJT130 | pET11a obgE | Tan et al., 2002 |
| pMK8 |
sulA::mCherry in a TGV light plasmid (from strain SS2514) |
S. Sandler |
| pSTL346 | cat araC obgE+ in pBAD33 | Foti et al., 2005 |
Strains isogenic with MG1655, except STL8248, used for ObgE protein purfication