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. 2009 Nov 12;4(11):e7794. doi: 10.1371/journal.pone.0007794

Figure 1. Kinetics of MDP-induced mRNA expression of TNF-α and IL-1β.

Figure 1

RT-qPCR of mRNA of TNF-α (A) and IL-1β (B). Kinetic studies of mRNA-response (C and D). Isolated monocytes were stimulated for 5 minutes (0.08 hours), 4 hours and 24 hours. Stimulation with MDP increased the level of mRNA transcription in monocytes from control subjects (p<0.01 and p<0.02 for TNF-α and IL-1β, respectively), whereas an upregulation was not seen in monocytes isolated from CARD15 non-mutated CD patients. Arbitrary units. Due to high variability in IL1β expression in both control and CD patients, these expression data were normalised to expression values at t = 0 hours. *p<0.05; **p<0.01.