Figure 2. MDP-induced expression IL-1β and activation of p38 and IKKα/β.

Immunoblotting of IL-1β (A), p38 (B), and IKKα/β (C). Quatitative IKKα/β phosphorylation, bars represent ranges (D). Monocytes were stimulated for 24 hours with MDP. IL-1β expression was increased in CD patients, but no response was seen to MDP. Monocytes stimulated with MDP had increased p38 phosphorylation, regardless of disease status. Contrary to the p38 response, reduced IKKα/β phosphorylation was seen in CD regardless of CARD15 status. Control monocytes did respond to MDP stimulation by increasing IKKα/β phosphorylation. CD: Crohn's disease. Crohn*: SNP8 homozygote.