Table 1. Occurrence in glomerular and tubular SAGE libraries from human kidneys of the specific tags of genes analyzed in this study.
G | PCT | cTAL | CCD | Tag sequence | |
Structure-specific markers | |||||
PODXL, Podocalyxin-like (NM_001018111) | 129 | 0 | 0 | 0 | ATATATGTCT |
WT1, Wilms tumor 1 (NM_000378) | 18 | 0 | 0 | 0 | TTACAAGATA |
ALDOB, Aldolase B (NM_000035) | 0 | 307 | 7 | 2 | AAATTTCACA |
SLC13A3, Solute carrier family 13 member 3 (NM_022829) | 0 | 71 | 0 | 0 | TGGGGTCTGT |
SLC12A1, Na/K/2Cl cotransporter 2 (NM_000338) | 0 | 0 | 149 | 1 | TGAGCAATCA |
UMOD, Uromodulin (NM_003361) | 3 | 3 | 837 | 4 | AATCCCGTGT |
AQP2, Aquaporin 2 (NM_000486) | 0 | 1 | 3 | 157 | ACACACACCA |
FXYD4, Corticosteroid hormone induced factor (NM_173160) | 0 | 2 | 0 | 191 | AGGAGGCTTC |
Ubiquitous reference genes | |||||
RPLP1, Ribosomal phosphoprotein, large, P1 (NM_001003) | 53 | 49 | 59 | 74 | AATGCCCTCA |
RPL19, Ribosomal protein L19 (NM_000981) | 124 | 98 | 75 | 83 | AGCCATTAAA |
PPIA, Peptidylpropyl isomerase A (NM_021130) | 8 | 12 | 17 | 20 | ATTTGGTGTG |
Genes selected for data standardization | |||||
DUSP9, Dual-specificity phosphatase 9 (NM_001395) | 0 | 1 | 30 | 0 | TATGCTTGTT |
GSTA1, Glutathione S-transferase α1 (NM_145740) | 1 | 43 | 4 | 0 | TGATGTGAAT |
KCNJ1, Renal outer-medullary potassium channel (NM_153767) | 0 | 3 | 27 | 23 | CCCACCTGCA |
MUC1, Mucin 1 (NM_002456) | 1 | 2 | 21 | 26 | CTGAACTGGA |
NPHS2, Podocin (NM_014625) | 68 | 0 | 0 | 0 | CCTCACTGAA |
PTGER1, Prostaglandin E receptor 1 (NM_000955) | 0 | 0 | 0 | 21 | CTGGACCCTT |
Pathology biomarkers | |||||
SERPINH1, Serpin peptidase inhibitor, H1 (NM_001235) | 1 | 1 | 0 | 2 | AAGCCTGCCT |
COL4A5, Collagen, type IV, alpha-5 (NM_000495) | 2 | 0 | 0 | 0 | AAGATAACAT |
CTGF, Connective tissue growth factor (NM_001901) | 239 | 2 | 4 | 5 | AGTTTTTTCA |
DCN, Decorin (NM_001920) | 107 | 0 | 0 | 0 | AAGTGACTTC |
TRPC6, Transient receptor potential cation channel C6 (NM_004621) | 1 | 0 | 0 | 0 | AGAAAATACA |
Data, from Chabardès-Garonne et al [11]. correspond to tag counts normalized to 50,000 total tags per library. Human Genome Organization (HUGO) gene symbol is followed by a usual name and the RefSeq identification. SAGE data are available at GEO (www.ncbi.nlm.nih.gov/geo/) (GSM10419, GSM10423, GSM10426 and GSM10428). Tag sequences are also provided.