Figure 3. Hemolytic activity of secreted factors from wild type V. cholerae and deletion mutant derivates.

Culture supernatants of C6706wt, ΔprtV, and ΔhapR were serially diluted and tested for hemolytic activity. (a) Bacteria grown in LB broth. (b) Bacteria grown in BHI broth. Bars and whiskers represent mean + 1SD of 3 independent experiments. Statistically significant changes from C6707wt at each dilution as estimated by one-way ANOVA are indicated. * p-value ≤0.05 and ** p-value <0.01.