Fig. 2. Endothelial dysfunction in blood vessels expressing Nox5.

The endothelium of mouse aorta was transduced via luminal delivery of control (β-gal/GFP) or Nox5 adenovirus (6×10¹¹ particles/ml). In A, en face presentation of a β-gal transduced aorta showing extent of endothelial cell uptake. In B, transduction of mouse aorta with Nox5 adenovirus increases superoxide production. In C, the relative expression of eNOS, phosphorylated eNOS and Nox5 (HA) in transduced vessels. In D, contractile responses to phenylephrine (PE, 10⁻⁹−10⁻⁵M) in blood vessels expressing Nox5 versus GFP. In E, vasorelaxant responses to acetylcholine (ACh, 10⁻⁹−10⁻⁵M). In F, vasorelaxation to sodium nitroprusside (SNP, 10⁻⁹−10⁻⁵M). Data is presented as mean force generated (2D) and as the means ± SE of the percentage of PE-induced tone (2E–F). *P<0.05 vs control vessels (n=5).