Table 1.
The averaged 18O/16O ratio from three individual LCMS runs for individual myoglobin peptides, organized by charge state
| Peptide | Number | Charge | Ratioa | Efficiency |
|---|---|---|---|---|
| Expected ratio of 1 | ||||
| LFTGHPETLEK | 4 | 2 | 1.24 | 0.76 |
| VEADIAGHGQEVLIR | 2 | 2 | 1.17 | 0.79 |
| VEADIAGHGQEVLIR | 1 | 3 | 0.74 | 0.90 |
| HGTVVLTALGGILK | 8 | 2 | 1.39 | 0.80 |
| ALELFR | 1 | 1 | 1.74 | 0.87 |
| YLEFISDAIIHVLHSK | 1 | 3 | 0.65 | 0.68 |
| protein mean | 1.15 | 0.80 | ||
| standard deviation | 0.41 | 0.08 | ||
| coefficient of variation | 0.35 | |||
| Expected ratio of 0.3 | ||||
| LFTGHPETLEK | 3 | 2 | 0.49 | 0.80 |
| HGTVVLTALGGILK | 3 | 2 | 0.40 | 0.72 |
| protein mean | 0.44 | 0.76 | ||
| standard deviation | 0.06 | 0.06 | ||
| coefficient of variation | 0.14 | |||
a
Ratios were generated using method 1 o f ZoomQuant. The data for the expected ratio of 1 were generated from three separate digests of myoglobin. The data for the 0.3 ratio is the average of 3 replicates from the same digest. “Number” indicates the number of times each peptide was observed within the data set, and “efficiency” indicates the percent of the peptide that was fully labeled relative to the sum of the fully labeled and singly labeled species.