Table 2.

The ¹⁸O/¹⁶O ratios calculated from zoom scans for the tryptic digest of the four protein mixture with an expected ¹⁸O/¹⁶O ratio of 1. Peptides that occur in multiple charge states are listed separately for each charge state. Ratios were generated using method 1 o f ZoomQuant

Peptide	Number	Charge	Ratio	Efficiency
Myoglobin
LFTGHPETELK	4	2	0.89	0.75
LFTGHPETELK	1	3	0.82	0.76
GLSDGEWQQVLNVWGK	2	2	1.00	0.49
VEADIAGHGQEVLIR	7	2	1.01	0.84
VEADIAGHGQEVLIR	3	3	1.06	0.66
HGTVVLTALGGILK	6	2	0.84	0.82
HGTVVLTALGGILK	1	3	0.82	0.85
YLEFISDAIIHVLHSK	2	2	0.51	0.81
YLEFISDAIIHVLHSK	1	3	0.61	0.20
protein mean			0.84	0.69
standard deviation			0.18	0.21
coefficient of variation			0.22
Protein Tyrosine Phosphatase - 1 β
LTISEDIK	4	2	0.57	0.47
FSYLAVIEGAK	6	2	0.90	0.84
FSYLAVIEGAK	1	1	0.84	0.82
ESGSLSPEHGPVVVHCSAGIGR	3	3	1.11	0.81
DVSPFDHSR	3	2	1.02	0.78
LHQEDNDYINASLIK	4	2	0.94	0.71
HEASDFPCRVAK	1	2	0.74	0.57
MGLIQTADQLR	1	2	0.97	0.90
protein mean			0.88	0.74
standard deviation			0.17	0.15
coefficient of variation			0.19
Glyceraldehyde-3-Phosphate Dehydrogenase
GAAQNIIPASTGAAK	4	2	1.06	0.83
VPTPNVSVVDLTCR	4	2	0.98	0.78
protein mean			1.02	0.80
standard deviation			0.05	0.03
coefficient of variation			0.05
Vascular Endothelial Growth Factor
HFLVQDPQCTCK	1	2	1.55	0.55
QLELNER	2	2	0.73	0.78
FMDVYQR	1	2	1.02	0.76
protein mean			1.10	0.70
standard deviation			0.58	0.17
coefficient of variation			0.52