Table 4.
The zoom scan to zoom scan variance of the myoglobin tryptic peptide HGTVVLTALGGILK. Sample numbers indicate individually performed tryptic digests of myoglobin. Ratios from individual zoom scans of the above peptide within a single LCMS run are listed. Replicates indicate repeated LCMS analysis for a single tryptic digest. The mean and standard deviation was calculated for each individual sample. Ratios were generated using method 1 of ZoomQuant
| Expected ratio of 1 |
Expected ratio of 0.3 |
Expected ratio of 3.0 |
|||||
|---|---|---|---|---|---|---|---|
| Myoglobin |
4 Protein mix |
Myoglobin |
4 Protein mix |
||||
| Obs. ratio | Obs. ratio | Obs. ratio | Obs. ratio | ||||
| Sample 1 | 1.69 | replicate 1 | 0.83 | replicate 1 | 0.40 | replicate 1 | 2.43 |
| 1.57 | 0.75 | replicate 2 | 0.48 | 3.36 | |||
| 1.68 | 0.96 | 0.32 | 2.60 | ||||
| Mean | 1.65 | 0.82 | mean | 0.41 | mean | 2.80 | |
| s.d. | 0.07 | mean | 0.84 | s.d. | 0.20 | s.d. | 0.50 |
| c.v. | 0.04 | s.d. | 0.09 | c.v. | 0.48 | c.v. | 0.18 |
| Sample 2 | 1.03 | c.v. | 0.10 | replicate 3 | 0.36 | replicate 2 | 2.24 |
| 1.30 | replicate 2 | 0.75 | 1.81 | ||||
| 1.00 | 0.75 | 2.66 | |||||
| 0.91 | mean | 0.75 | 1.70 | ||||
| Mean | 1.06 | s.d. | 0.01 | mean | 2.10 | ||
| s.d. | 0.17 | c.v. | 0.01 | s.d. | 0.44 | ||
| c.v. | 0.16 | replicate 3 | 0.99 | c.v. | 0.21 | ||
| Sample 3 | 1.98 | replicate 3 | 2.80 | ||||
| 2.74 | |||||||
| 2.25 | |||||||
| mean | 2.60 | ||||||
| s.d. | 0.30 | ||||||
| c.v. | 0.12 | ||||||