Table 5.
The calculated 18O/16O ratios from ten separate LCMS runs of the four standard protein tryptic digest were pooled. Samples that were mixed in 18O/16O ratios of 3 were adjusted to a value of one by multiplying by 0.3, and the standard deviation was calculated for each peptide
| Peptide | Number | Ratio | Std. dev. |
|---|---|---|---|
| ALELFR | 9 | 1.25 | 0.91 |
| DVSPFDHSR | 10 | 0.87 | 0.22 |
| ESGSLSPEHGPVVHCSAGIGR | 7 | 0.88 | 0.25 |
| FMDVYQR | 5 | 0.83 | 0.13 |
| FSYLAVIEGAK | 22 | 0.84 | 0.19 |
| GAAQNIIPASTGAAK | 15 | 1.04 | 0.41 |
| GLSDEWQQVLNVVWGK | 3 | 0.90 | 0.18 |
| HGTVVLTALGGILK | 23 | 0.84 | 0.13 |
| HLFVQDPQTCK | 8 | 1.91 | 0.79 |
| IVSNASCTTNCLAPLAK | 4 | 1.29 | 0.28 |
| LFTGHPETLEK | 13 | 1.20 | 0.56 |
| LFTGHPETLEKFDK | 2 | 0.66 | 0.23 |
| LHQEDNDYINASLIK | 11 | 0.95 | 0.23 |
| LTLISEDIK | 8 | 0.71 | 0.53 |
| MGLIQTADQLR | 3 | 0.75 | 0.19 |
| QLELNER | 5 | 1.21 | 0.84 |
| VEADIAGHGQEVLIR | 26 | 0.90 | 0.20 |
| VGVNGFGR | 2 | 0.81 | 0.20 |
| VPTPNSVSVVDLTCR | 9 | 1.08 | 0.49 |
| YLEFISDAIIHVLHSK | 5 | 0.48 | 0.12 |