Table 6.
Sequence coverage by protein was calculated for data acquired using the triple play MS method, and compared to sequence coverage when zoom scans were excluded from the MS method
| Myoglobin |
PTP-1β |
GAPDH |
VEGF |
|||||
|---|---|---|---|---|---|---|---|---|
| Sample | % a.a. | Peptides | % a.a. | Peptides | % a.a. | Peptides | % a.a. | Peptides |
| Expected 18O/16O ratio of 1 | ||||||||
| 6M urea replicate 1 | 39.87 | 6 | 6.21 | 1 | 18.67 | 5 | 8.41 | 2 |
| Replicate 2 | 35.29 | 5 | 2.5 | 1 | 9.34 | 2 | 5.14 | 1 |
| Replicate 3 | 50.33 | 7 | 4.6 | 2 | 15.96 | 4 | 9.47 | 2 |
| Mean | 41.83 | 6 | 4.43 | 1.33 | 14.65 | 3.66 | 7.67 | 1.66 |
| 8M urea replicate 1 | 38.56 | 4 | 13.1 | 4 | 4.22 | 1 | 3.27 | 1 |
| Replicate 2 | 49 | 6 | 25 | 8 | 8.73 | 2 | 8.41 | 2 |
| Replicate 3 | 36.7 | 4 | 16.78 | 5 | 8.73 | 2 | 6.54 | 2 |
| mean | 41.42 | 4.66 | 18.29 | 5.66 | 7.23 | 1.66 | 6.07 | 1.66 |
| Double play | 36.6 | 5 | 18.85 | 7 | 8.73 | 2 | 3.27 | 1 |
| Expected 18O/16O ratio of 3 | ||||||||
| 8 M urea replicate 1 | 32 | 5 | 22.76 | 5 | 8.73 | 3 | 3.27 | 3 |
| Replicate 2 | 30.07 | 5 | 20.69 | 7 | 8.73 | 2 | 5.14 | 1 |
| Replicate 3 | 32.03 | 4 | 22.76 | 6 | 8.73 | 2 | 3.27 | 1 |