Growth curve of hMSC and its derivatives and soft agar assay. A, growth curve of transfected cell lines. a, stripe-like pattern represented by MSC-TSR4, b, disorderly pattern represented by MSC-TSR6. B, anchorage independent growth in soft agar of transfected cell lines. a, MSC-T; b, MSC-S; c, MSC-TS; d, MSC-TSR4; e, MSC-TSR6; f, HOS. *, p<0.01 versus MSC-TS.