Fig. 4.
Growth kinetics of S. mutans UA159 strain containing the shuttle plasmid pDL277 (Leblanc et al., 1992) harbouring the CipB-encoding gene under the control of the raffinose-inducible promoter (notation ‘Pmsm’) of the S. mutans multiple-sugar metabolism operon (McLaughlin and Ferretti, 1996). Growth was monitored by following OD600 for 16 h in TYE containing either 0.5% raffinose (inducer, notation ‘raf’) or 0.5% glucose (repressor, notation ‘glc’). The vector alone (no insert) was used as control. Also plotted is the growth of a mutant defective in the putative immunity gene SMU.925 carrying the pMSM construct following induction with 0.5% raffinose.
