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. Author manuscript; available in PMC: 2010 May 1.
Published in final edited form as: Mol Microbiol. 2009 Apr 14;72(4):905–917. doi: 10.1111/j.1365-2958.2009.06693.x

Fig. 7.

Fig. 7

Transformation efficiency of S. mutans wild-type strain and its mutants deficient in the CipB bacteriocin and CipI immunity protein. Sheared genomic DNA carrying an antibiotic resistance gene was added alone or with 0.2 μM or 2 μM sCSP to growing cultures. Cells were grown for a further 2.5 h before differential plating. Results obtained for the wild-type strain showed that transformation is possible and equally efficient at the concentrations of sCSP that induce cell lysis. Mutants unable to undergo cell lysis (CipB–) showed no increase in transformation frequency in the presence of sCSP, while mutants with increased lysis potential (CipI–) showed increased transformation in the absence of sCSP. Transformation efficiencies are expressed as the number of antibiotic-resistant CFUs divided by the total number of CFUs. A ComE-deficient strain served as a transformation-deficient control.