Table 1.
Relative expression levels of highly expressed CSP-induced S. mutans genes encoding putative and known bacteriocins and their accessory genes.
| Locusa | Common name, putative function | Foldb |
|---|---|---|
| SMU.150 | NlmA mutacin IV | +11.19 |
| SMU.151 | NlmB mutacin IV | +12.40 |
| SMU.423 | Putative bacteriocin | +14.55 |
| SMU.925 | Putative immunity factor | +18.20 |
| SMU.1897 | ABC transporter, ATP-binding; ComA | +9.19 |
| SMU.1898 | Putative ABC transporter, ATP-binding and permease | +4.18 |
| SMU.1899 | Putative ABC transporter fragment | +5.22 |
| SMU.1900 | ABC transporter; ComB | +5.92 |
| SMU.1902 | GG-motif-containing peptide | +9.76 |
| SMU.1903 | Hypothetical protein | +15.97 |
| SMU.1904 | Hypothetical protein | +12.25 |
| SMU.1905 | GG-motif-containing peptide | +10.11 |
| SMU.1906 | Putative bacteriocin | +11.36 |
| SMU.1907 | Hypothetical protein | +8.93 |
| SMU.1908 | Hypothetical protein | +18.27 |
| SMU.1909 | Hypothetical protein | +19.61 |
| SMU.1910 | Hypothetical protein | +18.26 |
| SMU.1912 | Hypothetical protein | +22.23 |
| SMU.1913 | Putative immunity protein | +15.23 |
| SMU.1914 | Bacteriocin, mutacin V | +20.41 |
| SMU.1915 | Precursor of CSP pheromone | +3.76 |
| SMU.1916 | Histidine kinase ComD | +10.50 |
| SMU.1917 | Response regulator ComE | +11.32 |
| SMU.1997 | Sigma factor ComX | +14.27 |
a
Results for selected genes were ordered based on their position in the UA159 chromosome. The grey highlighted area indicates the genes located in a 13.5 kb bacteriocin-related genomic island. Putative bacteriocin-encoding genes are in bold.
b
Transcripts levels were measured by cDNA hybridized to a fourfold redundant S. mutans microarray and averaged for three replicated hybridizations. Quantitative real-time RT-PCR was performed on selected genes to confirm the results obtained using microarray.