Figure 3. IFN-γ-signaling is not required for AT-2 HIV-induced PDL-1, CD80 and CD86.

(A) Detection of IFN-γ by ELISA in supernatants from PBMC cultured in presence of AT-2 HIV or control microvescicles (Mock). (B) Surface expression of PDL-1, CD80 and CD86 analyzed by flow cytometry, gating on monocytes (CD14⁺ cells) or CCR5⁺ T cells (CD3⁺ cells), in PBMC cultured for 24 hours in presence of control microvescicles (Mock), AT-2 HIV alone or in presence of blocking antibodies against the cellular receptor for IFN-γ (anti-IFNGR), as well as rIFN-γ alone or in presence of anti-IFNGR. Flow cytometry histograms for one example characteristic of four independent experiments are shown.