Figure 5.

Distribution of experimentally determined Tn-PSM – SBA unbinding forces at increasing force loading rates. The data are collected from 1260 force curves obtained using six different tip retraction speeds in the interval 0.2 – 7 µm/s. The loading rate acting at a molecular bond was determined for each force jump from the slope Δf/Δt prior to each observed bond dissociation event. Based on the determined loading rate, the continuous distribution of observations was divided into 18 subgroups within a range of loading rates. The most probable unbinding force f^*, determined from the peak in the histograms as explained in figure 6 is visualized in the plot (large points).