Figure 2.

Expression of LRRK2 mutants in flies promotes DA neurodegeneration and concomitant locomotion deficits. A, B, Bar graph showing the number of TH-positive DA neurons in different clusters of various fly species at 20 or 60 d after eclosion, as indicated (n = 10). C, Representative confocal microscopy images showing TH-positive (red) DA neurons in the PPM1 cluster (boxed) of 60-d-old control or LRRK2-expressing flies. Inset, Higher magnification of boxed regions. D, E, Bar graph showing the percentage of various male (D) and female (E) flies at different days after eclosion that reached the top of assay column after 1 min (n = 20) (genotype: ddc–Gal4/+ or ddc–Gal4–hLRRK2).