FIGURE 4.

Effect of adoptive transfer of CD4⁺CD25⁺LAP⁺ and CD4⁺CD25⁺LAP⁻ cells on EAE. A, Schematic representation of experimental design. PT, Pertussis toxin. B, 1 × 10⁵ sorted CD4⁺CD25⁺LAP⁺ or CD4⁺CD25⁺LAP⁻ cells or PBS were injected i.v. into naive SJL mice. Mice were then immunized with PLP_139–151 in CFA to induce EAE 2 days after adoptive transfer. The mean daily score for each group (five mice per group) is shown. Data are representative of three experiments. C, Immunofluorescence microscopy of CD4⁺ cells in spinal cords. Cryosections of spinal cords from mice of control group (a) or mice receiving CD4⁺CD25⁺LAP⁺ cells (b) at the end of experiment were stained with anti-CD4. Results represent blinded analysis of three samples per group. ×400. Bar, 50 μm.