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Enzyme kinetics of wild type and N201Q
A. Michaelis-Menten plot of wild type and N201Q human α-NAGAL. Production of pNP from pNP-α-GalNAc was monitored by OD at 400nm. B. Summary of kinetic data on human α-NAGAL. The wild type and N201Q mutant glycoproteins have similar kinetic parameters. The specificity constant kcat/KM for each protein is 30-40 fold greater for the GalNAc substrate compared to the galactose substrate.
